Evaluating LAMP-based field diagnostics for the management of cassava mosaic disease in Lao PDR

Pinkham Vongphachanh

Abstract:

Cassava (Manihot esculenta, Crantz) is one of the most important staple food crops in Lao PDR after rice and Cassava mosaic disease (CMD) is a serious emerging threat to this crop. CMD can be caused by several species of begomoviruses but in Southeast Asia (SEA) Sri Lankan cassava mosaic virus (SLCMV) is the only virus species causing CMD in (SEA) reported so far.. Early detection of pathogen requires rapid detection protocols to confirm the disease symptoms observed, here we evaluated a new device which is portable and produce results in 1 hours. All leaf samples were first analyzed by PCR using primers specific for SLCMV and then by using the CMD field detection droid. The samples were taken from plants in field of Vientiane capital showing suspicious symptoms of CMD and include samples showing no symptoms The samples were macerated leaf tissue in extraction buffer and then extracted using dipsticks., The nucleic acids were then eluted directly into LAMP reaction mix and incubated into the Droid (8 samples for each time). The result returned in 60 min for accurate of identification all these samples with symptoms gave positive with the droid. The results confirm for the first time the occurrence of CMD and SLCMV in Vientiane, and the droid proved useful in the field. Currently we are evaluating its application in determining the quarantine state of the planting material before import within the country.
Keyword: Cassava (Manihot esculenta, Crantz), begomoviruses , Cassava mosaic disease, Sri Lankan cassava mosaic virus (SLCMV), Diagnostic droid


Biography:

Ms. Pinkham Vongphachanh she is a Plant Pathologist, she work at Plant Protection Center, Department of Agriculture, MOAF, she graduate master degree in 2016 her major is Plant pathology in Thailand. she started her work since 2007 to current her duties is assistant lab on Plant pathology diagnostic of fungi, bacteria and virus plant pathogens and preparation of specimens for herbarium collections, Participate in field surveys for the detection of plant pathogens, Trainer on Plant Protection to teacher training college of the north and the south in Lao PDR. Her research was focus on characterization of Rhizoctonia spp., complex isolated from sheath of rice in Lao PDR., Biological control of sheath disease of rice caused by Rhizoctonia oryzae and Rhizoctonia solani by Trichoderma spp. that published in Journal of Pure and Applied Microbiology and earlier topic on her research is identification of cassava mosaic disease and SriLankan cassava mosaic virus in Lao PDR, the result of this work has recently published in the The American Phytopathological Society (APS) in 2021.

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